24 US AI Startups That Have Raised $100M+ in 2025—So Far
The artificial intelligence boom in the United States is showing no signs of slowing down. After a record-breaking 2024, where 49 startups raised rounds of $100 million or more, 2025 is already shaping up to be another explosive year for AI innovation and investment.
As of mid-June, 24 U.S.-based AI startups have each raised at least $100 million in funding. Several firms have closed billion-dollar rounds, with major players like OpenAI and Anthropic continuing to dominate the AI landscape. But this year’s list also includes fresh entrants in AI infrastructure, healthcare, defense tech, and open-source platforms.
Below is a month-by-month breakdown of the standout funding rounds in 2025:
June
- Glean raised $150M Series F (Valuation: $7.25B) — Enterprise search
- Anysphere raised $900M Series C (Valuation: ~$10B) — AI coding tool Cursor
May
- Snorkel AI raised $100M Series D (Valuation: $1.3B) — AI data labeling
- LMArena raised $100M seed (Valuation: $600M) — AI model benchmarking
- TensorWave raised $100M Series A — AI infrastructure
April
- SandboxAQ raised $450M Series E (Valuation: $5.7B) — Quantum-AI hybrid
- Runway raised $308M Series D (Valuation: $3B) — AI video tools
March
- OpenAI raised $40B (Valuation: $300B) — Generative AI titan
- Nexthop AI raised $110M Series A — AI infrastructure
- Insilico Medicine raised $110M Series E (Valuation: $1B) — AI drug discovery
- Celestial AI raised $250M Series C (Valuation: $2.5B) — AI photonics
- Lila Sciences raised $200M seed — Science superintelligence
- Reflection.Ai raised $130M Series A (Valuation: $580M) — Autonomous systems
- Turing raised $111M Series E (Valuation: $2.2B) — LLM-integrated coding
- Shield AI raised $240M Series F (Valuation: $5.3B) — AI defense
- Anthropic raised $3.5B Series E (Valuation: $61.5B) — LLMs & safety
February
- Together AI raised $305M Series B (Valuation: $3.3B) — Open-source AI
- Lambda raised $480M Series D (Valuation: $2.5B) — AI hardware
- Abridge raised $250M Series D (Valuation: $2.75B) — Healthcare AI
- Eudia raised $105M Series A — Legal AI
- EnCharge AI raised $100M Series B — AI hardware
- Harvey raised $300M Series D (Valuation: $3B) — Legal tech AI
January
- ElevenLabs raised $180M Series C (Valuation: $3B+) — Synthetic voice
- Hippocratic AI raised $141M Series B (Valuation: $1.6B+) — LLMs for healthcare
AI Mega-Rounds Dominate 2025 VC Trends
This year’s data confirms that AI remains a magnet for capital, with multi-billion-dollar rounds increasingly common. OpenAI’s historic $40 billion round and Anthropic’s $3.5 billion Series E illustrate the intense interest in LLMs and foundational models.
But there’s also a surge in AI infrastructure, legal AI, healthcare, and scientific research startups, revealing investor appetite for deeper verticals beyond content generation and chatbots.
With another six months to go, 2025 could easily surpass last year’s record and cement the U.S. as the undisputed epicenter of AI venture funding.
